神戸大学附属図書館デジタルアーカイブ
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学内刊行物
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https://hdl.handle.net/20.500.14094/90008227
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2024-04-18
23:14 集計
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90008227 (fulltext)
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1.54 MB
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メタデータID
90008227
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy
著者
Sartsanga, Channarong ; Phengchat, Rinyaporn ; Fukui, Kiichi ; Wako, Toshiyuki ; Ohmido, Nobuko
著者名
Sartsanga, Channarong
著者名
Phengchat, Rinyaporn
著者名
Fukui, Kiichi
著者名
Wako, Toshiyuki
著者ID
A0558
研究者ID
1000030343263
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=489af0c45732b381520e17560c007669
著者名
Ohmido, Nobuko
近江戸, 伸子
オオミド, ノブコ
所属機関名
人間発達環境学研究科
収録物名
Chromosome Research
巻(号)
29(1)
ページ
81-94
出版者
Springer
刊行日
2021-03
公開日
2022-04-01
抄録
The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which consists of a DNA unit coiled around a core of histone molecules associated with linker histone. The compaction of chromatin fibers has been a topic of controversy since the discovery of chromosomes in the 19th century. Although chromatin fibers were first identified using electron microscopy, the chromatin fibers on the surface of chromosome structures in plants remain unclear due to shrinking and breaking caused by prior chromosome isolation or preparation with alcohol and acid fixation, and critical point drying occurred into dehydration and denatured chromosomal proteins. This study aimed to develop a high-quality procedure for the isolation and preparation of plant chromosomes, maintaining the native chromosome structure, to elucidate the organization of chromatin fibers on the surface of plant chromosomes by electron microscopy. A simple technique to isolate intact barley (Hordeum vulgare) chromosomes with a high yield was developed, allowing chromosomes to be observed with a high-resolution scanning ion microscopy and helium ion microscopy (HIM) imaging technology, based on a scanning helium ion beam. HIM images from the surface chromatin fibers were analyzed to determine the size and alignment of the chromatin fibers. The unit size of the chromatin fibers was 11.6 ± 3.5 nm and was closely aligned to the chromatin network model. Our findings indicate that compacting the surface structure of barley via a chromatin network and observation via HIM are powerful tools for investigating the structure of chromatin.
キーワード
chromatin fiber
helium ion microscopy (HIM)
Hordeum vuldare
plant chromosome
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature's AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s10577-021-09649-2
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資源タイプ
journal article
言語
English (英語)
ISSN
0967-3849
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eISSN
1573-6849
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関連情報
DOI
https://doi.org/10.1007/s10577-021-09649-2
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