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https://hdl.handle.net/20.500.14094/90008390
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2024-04-19
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90008390 (fulltext)
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メタデータID
90008390
アクセス権
open access
出版タイプ
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タイトル
Identification of a repressor for the two iol operons required for inositol catabolism in Geobacillus kaustophilus
著者
Yoshida, Ken-ichi ; Shirae, Yusuke ; Nishimura, Ryo ; Fukui, Kaho ; Ishikawa, Shu
著者ID
A1049
研究者ID
1000020230732
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=81199c8c7ea4889b520e17560c007669
著者名
Yoshida, Ken-ichi
吉田, 健一
ヨシダ, ケンイチ
所属機関名
科学技術イノベーション研究科
著者名
Shirae, Yusuke
著者名
Nishimura, Ryo
著者名
Fukui, Kaho
著者ID
A0019
研究者ID
1000030359872
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=ff864c575b0045a5520e17560c007669
著者名
Ishikawa, Shu
石川, 周
イシカワ, シュウ
所属機関名
科学技術イノベーション研究科
収録物名
Microbiology
巻(号)
167(1)
ページ
001008
出版者
Microbiology Society
刊行日
2021-01
公開日
2021-07-02
抄録
Geobacillus kaustophilus HTA426, a thermophilic Gram-positive bacterium, feeds on inositol as its sole carbon source, and an iol gene cluster required for inositol catabolism has been postulated with reference to the iol genes in Bacillus subtilis. The iol gene cluster of G. kaustophilus comprises two tandem operons induced in the presence of inositol; however, the mechanism underlying this induction remains unclear. B. subtilis iolQ is known to be involved in the regulation of iolX encoding scyllo-inositol dehydrogenase, and its homologue in HTA426 was found two genes upstream of the first gene (gk1899) of the iol gene cluster and was termed iolQ in G. kaustophilus. When iolQ was inactivated in G. kaustophilus, not only cellular myo-inositol dehydrogenase activity due to gk1899 expression but also the transcription of the two iol operons became constitutive. IolQ was produced and purified as a C-terminal histidine (His)-tagged fusion protein in Escherichia coli and subjected to an in vitro gel electrophoresis mobility shift assay to examine its DNA-binding property. It was observed that IolQ bound to the DNA fragments containing each of the two iol promoter regions and that DNA binding was antagonized by myo-inositol. Moreover, DNase I footprinting analyses identified two tandem binding sites of IolQ within each of the iol promoter regions. By comparing the sequences of the binding sites, a consensus sequence for IolQ binding was deduced to form a palindrome of 5′-RGWAAGCGCTTSCY-3′ (where R=A or G, W=A or T, S=G or C, and Y=C or T). IolQ functions as a transcriptional repressor regulating the induction of the two iol operons responding to myo-inositol.
キーワード
Geobacillus
Gram-positive
inositol
repressor
thermophile
カテゴリ
科学技術イノベーション研究科
学術雑誌論文
権利
© 2021 The Authors.
This is an Open Access article published by the Microbiology Society under the Creative Commons Attribution License
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資源タイプ
journal article
言語
English (英語)
ISSN
1350-0872
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eISSN
1465-2080
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NCID
AA10958193
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関連情報
DOI
https://doi.org/10.1099/mic.0.001008
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