神戸大学附属図書館デジタルアーカイブ
入力補助
English
カテゴリ
学内刊行物
ランキング
アクセスランキング
ダウンロードランキング
https://hdl.handle.net/20.500.14094/90008430
このアイテムのアクセス数:
20
件
(
2024-04-26
07:43 集計
)
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
90008430 (fulltext)
pdf
18.4 MB
7
メタデータ
ファイル出力
メタデータID
90008430
アクセス権
open access
出版タイプ
Version of Record
タイトル
Tongue Cancer Cell-Derived CCL20 Induced by Interaction With Macrophages Promotes CD163 Expression on Macrophages
著者
Shigeoka, Manabu ; Koma, Yu-ichiro ; Kodama, Takayuki ; Nishio, Mari ; Akashi, Masaya ; Yokozaki, Hiroshi
著者ID
A0858
研究者ID
1000020778716
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=4f6fddbc6f141752520e17560c007669
著者名
Shigeoka, Manabu
重岡, 学
シゲオカ, マナブ
所属機関名
医学研究科
著者ID
A0831
研究者ID
1000040714647
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=e984dab156ad8106520e17560c007669
著者名
Koma, Yu-ichiro
狛, 雄一朗
コマ, ユウイチロウ
所属機関名
医学研究科
著者名
Kodama, Takayuki
著者ID
A0873
研究者ID
1000000781824
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=be31fb9c73d7149a520e17560c007669
著者名
Nishio, Mari
西尾, 真理
ニシオ, マリ
所属機関名
医学研究科
著者ID
A1492
研究者ID
1000040597168
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=b7649a2950dc725a520e17560c007669
著者名
Akashi, Masaya
明石, 昌也
アカシ, マサヤ
所属機関名
医学研究科
著者ID
A1660
研究者ID
1000010200891
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=f5b3e42ac5b81cad520e17560c007669
著者名
Yokozaki, Hiroshi
横崎, 宏
ヨコザキ, ヒロシ
所属機関名
医学研究科
収録物名
Frontiers in Oncology
巻(号)
11
ページ
667174
出版者
Frontiers Media
刊行日
2021-06-09
公開日
2021-07-13
抄録
Background: CD163-positive macrophages contribute to the aggressiveness of oral squamous cell carcinoma. We showed in a previous report that CD163-positive macrophages infiltrated not only to the cancer nest but also to its surrounding epithelium, depending on the presence of stromal invasion in tongue carcinogenesis. However, the role of intraepithelial macrophages in tongue carcinogenesis remains unclear. In this study, we assessed the biological behavior of intraepithelial macrophages on their interaction with cancer cells. Materials and Methods: We established the indirect coculture system (intraepithelial neoplasia model) and direct coculture system (invasive cancer model) of human monocytic leukemia cell line THP-1-derived CD163-positive macrophages with SCC25, a tongue squamous cell carcinoma (TSCC) cell line. Conditioned media (CM) harvested from these systems were analyzed using cytokine array and enzyme-linked immunosorbent assay and extracted a specific upregulated cytokine in CM from the direct coculture system (direct CM). The correlation of both this cytokine and its receptor with various clinicopathological factors were evaluated based on immunohistochemistry using clinical samples from 59 patients with TSCC. Moreover, the effect of this cytokine in direct CM on the phenotypic alterations of THP-1 was confirmed by real-time polymerase chain reaction, western blotting, immunofluorescence, and transwell migration assay. Results: It was shown that CCL20 was induced in the direct CM specifically. Interestingly, CCL20 was produced primarily in SCC25. The expression level of CCR6, which is a sole receptor of CCL20, was higher than the expression level of SCC25. Our immunohistochemical investigation showed that CCL20 and CCR6 expression was associated with lymphatic vessel invasion and the number of CD163-positive macrophages. Recombinant human CCL20 induced the CD163 expression and promoted migration of THP-1. We also confirmed that a neutralizing anti-CCL20 antibody blocked the induction of CD163 expression by direct CM in THP-1. Moreover, ERK1/2 phosphorylation was associated with the CCL20-driven induction of CD163 expression in THP-1. Conclusions: Tongue cancer cell-derived CCL20 that was induced by interaction with macrophages promotes CD163 expression on macrophages.
キーワード
tongue cancer
CCL20
cancer microenvironment
macrophage
CD163
cell-cell interaction
カテゴリ
医学研究科
学術雑誌論文
権利
© 2021 Shigeoka, Koma, Kodama, Nishio, Akashi and Yokozaki.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
詳細を表示
資源タイプ
journal article
言語
English (英語)
eISSN
2234-943X
OPACで所蔵を検索
CiNiiで学外所蔵を検索
関連情報
DOI
https://doi.org/10.3389/fonc.2021.667174
ホームへ戻る