神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90008702
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2024-04-21
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90008702 (fulltext)
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90008702
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open access
出版タイプ
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タイトル
Complex Organ Construction from Human Pluripotent Stem Cells for Biological Research and Disease Modeling with New Emerging Techniques
著者
Matsumoto, Ryusaku ; Yamamoto, Takuya ; Takahashi, Yutaka
著者名
Matsumoto, Ryusaku
著者名
Yamamoto, Takuya
著者ID
A0765
研究者ID
1000070301281
著者名
Takahashi, Yutaka
高橋, 裕
タカハシ, ユタカ
所属機関名
医学研究科
収録物名
International Journal of Molecular Sciences
巻(号)
22(19)
ページ
10184
出版者
MDPI
刊行日
2021-10
公開日
2021-10-28
抄録
Human pluripotent stem cells (hPSCs) are grouped into two cell types; embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs). hESCs have provided multiple powerful platforms to study human biology, including human development and diseases; however, there were difficulties in the establishment of hESCs from human embryo and concerns over its ethical issues. The discovery of hiPSCs has expanded to various applications in no time because hiPSCs had already overcome these problems. Many hPSC-based studies have been performed using two-dimensional monocellular culture methods at the cellular level. However, in many physiological and pathophysiological conditions, intra- and inter-organ interactions play an essential role, which has hampered the establishment of an appropriate study model. Therefore, the application of recently developed technologies, such as three-dimensional organoids, bioengineering, and organ-on-a-chip technology, has great potential for constructing multicellular tissues, generating the functional organs from hPSCs, and recapitulating complex tissue functions for better biological research and disease modeling. Moreover, emerging techniques, such as single-cell transcriptomics, spatial transcriptomics, and artificial intelligence (AI) allowed for a denser and more precise analysis of such heterogeneous and complex tissues. Here, we review the applications of hPSCs to construct complex organs and discuss further prospects of disease modeling and drug discovery based on these PSC-derived organs.
キーワード
pluripotent stem cell
cellular heterogeneity
cell-cell interaction
organoid
bioengineering
organ on a chip
single-cell transcriptomics
spatial transcriptomics
artificial intelligence
カテゴリ
医学研究科
学術雑誌論文
権利
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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journal article
言語
English (英語)
eISSN
1422-0067
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NCID
AA12038549
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DOI
https://doi.org/10.3390/ijms221910184
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