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https://hdl.handle.net/20.500.14094/90008933
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2024-04-27
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90008933 (fulltext)
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90008933
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open access
出版タイプ
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タイトル
Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation
著者
Kawaguchi, Hideo ; Hasunuma, Tomohisa ; Ohnishi, Yasuo ; Sazuka, Takashi ; Kondo, Akihiko ; Ogino, Chiaki
著者ID
A0136
研究者ID
1000050463873
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=28b260930fa04130520e17560c007669
著者名
Kawaguchi, Hideo
川口, 秀夫
カワグチ, ヒデオ
所属機関名
科学技術イノベーション研究科
著者ID
A0960
研究者ID
1000020529606
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=73b63639d47d0a4b520e17560c007669
著者名
Hasunuma, Tomohisa
蓮沼, 誠久
ハスヌマ, トモヒサ
所属機関名
先端バイオ工学研究センター
著者名
Ohnishi, Yasuo
著者名
Sazuka, Takashi
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
著者ID
A0339
研究者ID
1000000313693
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=cb7c84b4e252c367520e17560c007669
著者名
Ogino, Chiaki
荻野, 千秋
オギノ, チアキ
所属機関名
工学研究科
収録物名
Microbial Cell Factories
巻(号)
20(1)
ページ
228
出版者
BMC
刊行日
2021-12-23
公開日
2022-01-19
抄録
Background: Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics. Results: Under aerobic conditions, a recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI genes derived from Streptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. An ldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture. Conclusions: Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives.
キーワード
Corynebacterium glutamicum
Dissolved oxygen
Metabolic engineering
Metabolome analysis
Amino acid
カテゴリ
科学技術イノベーション研究科
工学研究科
先端バイオ工学研究センター
学術雑誌論文
権利
© The Author(s) 2021. Open Access
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
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資源タイプ
journal article
言語
English (英語)
eISSN
1475-2859
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NCID
AA12039472
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関連情報
DOI
https://doi.org/10.1186/s12934-021-01714-z
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