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https://hdl.handle.net/20.500.14094/90009064
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2024-04-27
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90009064 (fulltext)
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90009064
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open access
出版タイプ
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タイトル
Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain
著者
Kobayashi, Yuma ; Inokuma, Kentaro ; Matsuda, Mami ; Kondo, Akihiko ; Hasunuma, Tomohisa
著者名
Kobayashi, Yuma
著者ID
A1270
研究者ID
1000090532606
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=30156c40b4d023df520e17560c007669
著者名
Inokuma, Kentaro
猪熊, 健太郎
イノクマ, ケンタロウ
所属機関名
科学技術イノベーション研究科
著者ID
A2801
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=308d4779b81c47c0520e17560c007669
著者名
Matsuda, Mami
松田, 真実
マツダ, マミ
所属機関名
科学技術イノベーション研究科
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
著者ID
A0960
研究者ID
1000020529606
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=73b63639d47d0a4b520e17560c007669
著者名
Hasunuma, Tomohisa
蓮沼, 誠久
ハスヌマ, トモヒサ
所属機関名
先端バイオ工学研究センター
収録物名
Metabolic Engineering Communications
巻(号)
13
ページ
e00188
出版者
Elsevier B.V.
刊行日
2021-12
公開日
2022-03-15
抄録
Resveratrol is a plant-derived aromatic compound with a wide range of beneficial properties including antioxidant and anti-aging effects. The resveratrol currently available on the market is predominantly extracted from certain plants such as grape and the Japanese knotweed Polygonum cuspidatum. Due to the unstable harvest of these plants and the low resveratrol purity obtained, it is necessary to develop a stable production process of high-purity resveratrol from inexpensive feedstocks. Here, we attempted to produce resveratrol from a wide range of sugars as carbon sources by a using the genetically-engineered yeast Scheffersomyces stipitis (formerly known as Pichia stipitis), which possesses a broad sugar utilization capacity. First, we constructed the resveratrol producing strain by introducing genes coding the essential enzymes for resveratrol biosynthesis [tyrosine ammonia-lyase 1 derived from Herpetosiphon aurantiacus (HaTAL1), 4-coumarate: CoA ligase 2 derived from Arabidopsis thaliana (At4CL2), and stilbene synthase 1 derived from Vitis vinifera (VvVST1)]. Subsequently, a feedback-insensitive allele of chorismate mutase was overexpressed in the constructed strain to improve resveratrol production. The constructed strain successfully produced resveratrol from a broad range of biomass-derived sugars [glucose, fructose, xylose, N-acetyl glucosamine (GlcNAc), galactose, cellobiose, maltose, and sucrose] in shake flask cultivation. Significant resveratrol titers were detected in cellobiose and sucrose fermentation (529.8 and 668.6 mg/L after 120 h fermentation, respectively), twice above the amount obtained with glucose (237.6 mg/L). Metabolomic analysis revealed an altered profile of the metabolites involved in the glycolysis and shikimate pathways, and also of cofactors and metabolites of energy metabolisms, depending on the substrate used. The levels of resveratrol precursors such as L-tyrosine increased in cellobiose and sucrose-grown cells. The results indicate that S. stipitis is an attractive microbial platform for resveratrol production from broad types of biomass-derived sugars and the selection of suitable substrates is crucial for improving resveratrol productivity of this yeast.
キーワード
Scheffersomyces stipitis
Non-conventional yeast
Resveratrol
Metabolic engineering
Shikimate pathway
Disaccharide dissimilation
カテゴリ
科学技術イノベーション研究科
先端バイオ工学研究センター
学術雑誌論文
権利
© 2021 The Authors. Published by Elsevier B.V. on behalf of International Metabolic Engineering Society.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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資源タイプ
journal article
言語
English (英語)
eISSN
2214-0301
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関連情報
DOI
https://doi.org/10.1016/j.mec.2021.e00188
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