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https://hdl.handle.net/20.500.14094/90009215
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2024-04-26
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90009215 (fulltext)
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90009215
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open access
出版タイプ
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タイトル
Pulsed-Ultrasound Irradiation Induces the Production of Itaconate and Attenuates Inflammatory Responses in Macrophages
著者
Yamaguchi, Atomu ; Maeshige, Noriaki ; Ma, Xiaoqi ; Uemura, Mikiko ; Noguchi, Hikari ; Matsuda, Mami ; Nishimura, Yuya ; Hasunuma, Tomohisa ; Kondo, Hiroyo ; Fujino, Hidemi
著者名
Yamaguchi, Atomu
著者ID
A1349
研究者ID
1000090617838
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=bad2e50ccfd61e15520e17560c007669
著者名
Maeshige, Noriaki
前重, 伯壮
マエシゲ, ノリアキ
所属機関名
保健学研究科
著者名
Ma, Xiaoqi
著者名
Uemura, Mikiko
著者名
Noguchi, Hikari
著者ID
A2801
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=308d4779b81c47c0520e17560c007669
著者名
Matsuda, Mami
松田, 真実
マツダ, マミ
所属機関名
科学技術イノベーション研究科
著者ID
A1345
研究者ID
1000040728218
著者名
Nishimura, Yuya
西村, 勇哉
ニシムラ, ユウヤ
所属機関名
科学技術イノベーション研究科
著者ID
A0960
研究者ID
1000020529606
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=73b63639d47d0a4b520e17560c007669
著者名
Hasunuma, Tomohisa
蓮沼, 誠久
ハスヌマ, トモヒサ
所属機関名
先端バイオ工学研究センター
著者名
Kondo, Hiroyo
著者ID
A1223
研究者ID
1000020278998
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=becd953e41b2f244520e17560c007669
著者名
Fujino, Hidemi
藤野, 英己
フジノ, ヒデミ
所属機関名
保健学研究科
収録物名
Journal of Inflammation Research
巻(号)
15
ページ
2387-2395
出版者
Dove Medical Press
刊行日
2022-04-13
公開日
2022-05-17
抄録
Background: Itaconate is a key metabolite in the innate immune system and exerts strong anti-inflammatory effects in macrophages. For the production of itaconate in macrophages, immune-responsive gene 1 (IRG1) is an imperative enzyme, and activating the IRG1-itaconate pathway is reported to alleviate inflammatory diseases by upregulating nuclear factor-erythroid 2-related factor 2 (NRF2). However, there are very few reports on strategies to increase itaconate production. Ultrasound therapy is a widely used intervention for anti-inflammatory and soft-tissue regeneration purposes. Here we show the effect of ultrasound irradiation on the production of itaconate in macrophages. Methods: Murine bone marrow-derived macrophages (BMDMs) were exposed to pulsed ultrasound (3.0 W/cm2) for 5 minutes. Three hours after irradiation, the intracellular levels of metabolites and mRNA expression levels of Irg1 and Nrf2 were measured using CE/MS and qPCR, respectively. To evaluate macrophage inflammation status, 3 h after irradiation, the cells were stimulated with 100 ng/mL lipopolysaccharide (LPS) for 1.5 h and the mRNA expression levels of pro-inflammatory factors (Il-1β, Il-6, and Tnf-α) were measured. Student's t-test, one-way ANOVA and Tukey's multiple comparison test were used for statistical processing, and the significance level was set to less than 5%. Results: Ultrasound irradiation significantly increased the intracellular itaconate level and the expression levels of Irg1 and Nrf2 in BMDMs. Upregulation of Il-1β, Il-6, and Tnf-α by LPS was significantly suppressed in BMDMs treated with ultrasound. Ultrasound irradiation did not affect cell viability and apoptosis. Conclusion: Ultrasound irradiation induces the production of itaconate by upregulating Irg1 expression and attenuates inflammatory responses in macrophages via Nrf2. These results suggest that ultrasound is a potentially useful method to increase itaconate production in macrophages.
キーワード
itaconate
IRG1
NRF2
pulsed-ultrasound
macrophage
inflammation
カテゴリ
科学技術イノベーション研究科
先端バイオ工学研究センター
保健学研究科
学術雑誌論文
権利
© 2022 Yamaguchi et al. This work is published and licensed by Dove Medical Press Limited.
The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
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資源タイプ
journal article
言語
English (英語)
eISSN
1178-7031
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関連情報
DOI
https://doi.org/10.2147/JIR.S361609
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