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https://doi.org/10.24546/00098307
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2024-04-25
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00098307 (fulltext)
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メタデータID
00098307
アクセス権
open access
出版タイプ
Version of Record
タイトル
Interleukin-6 Facilitates Escherichia coli Transcytosis in the Caco-2 Cell Monolayer System
著者
Usami, Makoto ; Matsushita, Emi ; Domori, Miho ; Inoue, Yumiko ; Miki, Akinori ; Kataoka, Nobumasa ; Tabuchi, Yoshiki ; Sekita, Mikio
著者名
Usami, Makoto
著者名
Matsushita, Emi
著者名
Domori, Miho
著者名
Inoue, Yumiko
著者名
Miki, Akinori
著者名
Kataoka, Nobumasa
著者名
Tabuchi, Yoshiki
著者名
Sekita, Mikio
収録物名
Bulletin of health sciences Kobe
巻(号)
15
ページ
35-43
出版者
Graduate School of Health Sciences, Kobe University
刊行日
1999-12-26
公開日
2006-09-11
注記
本文ファイルは、国立情報学研究所CiNiiより提供されたものです。
抄録
Aim : Bacterial translocation is a major concern as a cause developing sepsis or multiple organ failure under systemic inflammatory response with hypercytokinemia aroused from surgical stress. However, direct effect of interleukin 6 (IL-6) on bacterial translocation has rarely been reported. In this study, the effect of IL-6 on enterocyte with enterononinvasive E. coli interaction was evaluated in the intestinal epithelial cells. Methods : Direct effect of IL-6 on matured villus enterocytes, confluent Caco-2 cells on porous filter with double chamber system (Transwell), was evaluated by measuring permeability from apical (intestinal lumen side) to basolateral chamber (blood vessel side). Viable E. coli c-25 permeability, fluorescein sulfonic acid permeability, and transepithelial electrical resistance, LDH secretion, and ultrastructural analysis were performed under 100 ng/ml of IL-6 in the basolateral chamber medium. Results: IL-6 directly stimulated E. coli passage across Caco-2 monolayer cells by transcellular route with intact nuclear and cytoplasmic structure. Paracellular permeability change and cellular injury were not observed. It is suggested that IL-6 stimulates enterocytes from basolateral side, affects enterocyte-E. coli interaction, and increases E. coli transcytosis. Conclusions : IL-6 directly facilitates E. coli transcytosis in monolayer intestinal cells.
キーワード
bacterial translocation
Caco-2 cell
IL-6
Escherichia coli
transcellular pathway
カテゴリ
Bulletin of health sciences Kobe
>
15号(1999-12-26)
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資源タイプ
departmental bulletin paper
言語
English (英語)
ISSN
1346-8707
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NCID
AA11316362
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関連情報
NAID
110004599672
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