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https://doi.org/10.24546/00098335
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2024-04-26
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00098335 (fulltext)
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メタデータID
00098335
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open access
出版タイプ
Version of Record
タイトル
Effect of γ-Linolenic Acid (GLA) or Docosahexaenoic Acid (DHA) on Tight Junction Permeability in Intestinal Monolayer Cells
著者
Usami, Makoto ; Komurasaki, Takako ; Hanada, Aki ; Kinoshita, Kaori ; Ohata, Atsushi ; Tabuchi, Yoshiki ; Saitoh, Masaki ; Nakagawa, Masashi
著者名
Usami, Makoto
著者名
Komurasaki, Takako
著者名
Hanada, Aki
著者名
Kinoshita, Kaori
著者名
Ohata, Atsushi
著者名
Tabuchi, Yoshiki
著者名
Saitoh, Masaki
著者名
Nakagawa, Masashi
収録物名
Bulletin of health sciences Kobe
巻(号)
17
ページ
63-72
出版者
Graduate School of Health Sciences, Kobe University
刊行日
2001-11-30
公開日
2006-09-11
注記
本文ファイルは、国立情報学研究所CiNiiより提供されたものです。
抄録
Polyunsaturated fatty acids have been characterized as immunonutrients, however the effect of docosahexaenoic acid (DHA) or γ-linolenic acid (GLA) on intestinal permeability has rarely been reported, Confluent Caco-2 cells on porous filter were used to measure tight junction function by fluorescein sulfonic acid (FS) permeability and transepithelial electrical resistance (TEER). Treatment of 0, 10, 50, 100 , μM of DHA or GLA during 24 hours were compared. Then, effects of butylated hydroxytoluene (BHT, antioxidant), H7 (protein kinase C antagonist), or inhibitors of enzymatic degradation to the eicosanoids, indomethacin (cyclooxygenase inhibitor), AA861 (lipoxygenase inhibitor) on DHA or GLA were examined. GLA and DHA enhanced FS permeability to 8.7 and 1.4 fold and lowered TEER to 0.52 and 0.73 fold vs. control with concentration dependently without cell injury (p<0.01-0.05). IND and AA861 enhanced the changes mediated by GLA but did not alter DHA effect. BHT was ineffective. H7 facilitated the changes mediated by GLA, DHA, and eicosapentaenoic acid (EPA). It is indicated that the mechanism to change tight junction permeability via protein kinase C regulation is common but via eicosanoids formation is different among GLA, DHA and EPA. In conclusion, GLA and DHA affect tight junction permeability in intestinal monolayer cells specifically and concentration dependently.
キーワード
tight junction permeability
Caco-2 cell
docosahexaenoic acid
γ-linolenic acid
eicosapentaenoic acid
protein kinase C
indomethacin
AA861
カテゴリ
Bulletin of health sciences Kobe
>
17号(2001-11-30)
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資源タイプ
departmental bulletin paper
言語
English (英語)
ISSN
1346-8707
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NCID
AA11316362
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関連情報
NAID
110004599722
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