| 本文URL |  ダウンロード回数： 回 |
| ID | 90004192 |
| 資源タイプ | |
| 版区分 | publisher |
| タイトル | Taurine does not affect the composition, diversity, or metabolism of human colonic microbiota simulated in a single-batch fermentation system |
| 著者ID | A0278 / A0276 / A1291 / A1329 / / A1053 / A1049 / A1715 / A1682 |
| 著者 | |
| 著者別表記 | 佐々木, 建吾 / 佐々木, 大介 / 岡井, 直子 / 田中, 耕生 / / 福田, 伊津子 / 吉田, 健一 / 近藤, 昭彦 / 大澤, 朗 |
| 著者ヨミ | |
| 著者所属 | |
| 掲載誌 | |
| 巻号 | 12(7) |
| ページ | e0180991 |
| 公開者 | Public Library of Science |
| 刊行日 | 2017-07-10 |
| 権利 | © 2017 Sasaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 公開日 | 2017-09-22 |
| eISSN | 19326203 |
| DOI | 10.1371/journal.pone.0180991 |
| 言語 | eng |
| 研究者ID | 1000050558301 / 1000000650615 / 1000020542137 / 1000050418696 / / 1000050418943 / 1000020230732 / 1000040205547 / 1000010253189 |
| 内容記述 | Accumulating evidence suggests that dietary taurine (2-aminoethanesulfonic acid) exerts beneficial anti-inflammatory effects in the large intestine. In this study, we investigated the possible impact of taurine on human colonic microbiota using our single-batch fermentation system (Kobe University Human Intestinal Microbiota Model; KUHIMM). Fecal samples from eight humans were individually cultivated with and without taurine in the KUHIMM. The results showed that taurine remained largely undegraded after 30 h of culturing in the absence of oxygen, although some 83% of the taurine was degraded after 30 h of culturing under aerobic conditions. Diversity in bacterial species in the cultures was analyzed by 16S rRNA gene sequencing, revealing that taurine caused no significant change in the diversity of the microbiota; both operational taxonomic unit and Shannon-Wiener index of the cultures were comparable to those of the respective source fecal samples. In addition, principal coordinate analysis indicated that taurine did not alter the composition of bacterial species, since the 16S rRNA gene profile of bacterial species in the original fecal sample was maintained in each of the cultures with and without taurine. Furthermore, metabolomic analysis revealed that taurine did not affect the composition of short-chain fatty acids produced in the cultures. These results, under these controlled but artificial conditions, suggested that the beneficial anti-inflammatory effects of dietary taurine in the large intestine are independent of the intestinal microbiota. We infer that dietary taurine may act directly in the large intestine to exert anti-inflammatory effects. |
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| データ登録日 | 2017-09-22 |
