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Construction of a Model Culture System of Human Colonic Microbiota to Detect Decreased Lachnospiraceae Abundance and Butyrogenesis in the Feces of Ulcerative Colitis Patients

A0278 / A0870 / A0276 / A0828 / / A1053 / A0058 / A1715 / A1682

Sasaki, Kengo
Inoue, Jun
Sasaki, Daisuke
Hoshi, Namiko
Shirai, Tomokazu
Fukuda, Itsuko
Azuma, Takeshi
Kondo, Akihiko
Osawa, Ro

佐々木, 建吾 / 井上, 潤 / 佐々木, 大介 / 星, 奈美子 / / 福田, 伊津子 / 東, 健 / 近藤, 昭彦 / 大澤, 朗

14(5)

1800555

Wiley‐VCH Verlag

2019-05

© 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: [Biotechnology Journal, 14(5): 1800555, 2019], which has been published in final form at https://doi.org/10.1002/biot.201800555. This article may be used for non-commercial purposes in accordance with Wiley-VCH Terms and Conditions for Use of Self-Archived Versions.

2020-06-01

18607314

eng

1000050558301 / 1000050631561 / 1000000650615 / 1000040645214 / / 1000050418943 / 1000060221040 / 1000040205547 / 1000010253189

Compositional alteration of the gut microbiota is associated with ulcerative colitis (UC). Here, a model culture system is established for the in vitro human colonic microbiota of UC, which will be helpful for determining medical interventions. 16S ribosomal RNA sequencing confirms that UC models are successfully developed from fecal inoculum and retain the bacterial species biodiversity of UC feces. The UC models closely reproduce the microbial components and successfully preserve distinct clusters from the healthy subjects (HS), as observed in the feces. The relative abundance of bacteria belonging to the family Lachnospiraceae significantly decreases in the UC models compared to that in HS, as observed in the feces. The system detects significantly lower butyrogenesis in the UC models than that in HS, correlating with the decreased abundance of Lachnospiraceae. Interestingly, the relative abundance of Lachnospiraceae does not correlate with disease activity (defined as partial Mayo score), suggesting that Lachnospiraceae persists in UC patients at a decreased level, irrespective of the alteration in disease activity. Moreover, the system shows that administration of Clostridium butyricum MIYAIRI restores butyrogenesis in the UC model. Hence, the model detects deregulation in the intestinal environment in UC patients and may be useful for simulating the effect of probiotics.

butyrate / microbiota / model culture systems / probiotics / ulcerative colitis

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2019-06-20