本文URL ダウンロード回数：
回
ID 90006833
資源タイプ 版区分 publisher
タイトル Level of Seven Neuroblastoma-Associated mRNAs Detected by Droplet Digital PCR Is Associated with Tumor Relapse/Regrowth of High-Risk Neuroblastoma Patients
著者ID / / A2570 / A0844 / / / / / / A1395 / / / / A2317 / A0877 / A1683
著者 Thwin, Khin K. M.
Ishida, Toshiaki
Uemura, Suguru
Yamamoto, Nobuyuki
Lin, Kyaw S.
Tamura, Akihiro
Kozaki, Aiko
Saito, Atsuro
Kishimoto, Kenji
Mori, Takeshi
Hasegawa, Daiichiro
Kosaka, Yoshiyuki
Nino, Nanako
Takafuji, Satoru
Iijima, Kazumoto
Nishimura, Noriyuki
著者別表記 / / 植村, 優 / 山本, 暢之 / / / / / / 森, 健 / / / / 髙藤, 哲 / 飯島, 一誠 / 西村, 範行
著者ヨミ 著者所属 掲載誌 巻号 22(2)
ページ 236-246
公開者 Elsevier
刊行日 2020-02
権利 © 2020 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0).
公開日 2020-03-09
ISSN 15251578 eISSN 19437811
NCID AA11395041 DOI 10.1016/j.jmoldx.2019.10.012 資源情報1 言語 eng
研究者ID / / 1000040814348 / 1000020596043 / / / / / / 1000080403236 / / / / 1000070816800 / 1000000240854 / 10000322719
内容記述 Monitoring of several sets of neuroblastoma-associated mRNAs (NB-mRNAs) by real-time quantitative PCR (qPCR) can be used to evaluate minimal residual disease in NB patients. Droplet digital PCR (ddPCR) is an adaption of qPCR that potentially provides simpler and more reproducible detection of low levels of mRNAs. However, whether minimal residual disease in NB patients can be monitored by ddPCR using a set of NB-mRNAs is not yet tested. In this study, 208 bone marrow (BM) and 67 peripheral blood samples were retrospectively collected from 20 high-risk NB patients with clinical disease evaluation at two Japanese centers between 2011 and 2018, and level of each NB-mRNA (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNAs) was determined by ddPCR. Level of 7NB-mRNAs (defined as the combined signature of each NB-mRNA) was higher in BM than peripheral blood, but correlated significantly with each other. In accordance with disease burden, it varied with disease status (remission, stable, or progression) and collection time point (diagnosis, treatment, post-treatment, or relapse). In 73 post-treatment BM samples, it was significantly higher in 17 relapsed/regrown samples than in 56 nonrelapsed/nonregrown samples. Furthermore, ddPCR had a better prognostic value than qPCR in detecting 7NB-mRNAs in the same 73 post-treatment BM samples. This study suggests that ddPCR detection of 7NB-mRNAs is significantly associated with tumor relapse/regrowth in high-risk NB patients.
KUID / / / https://kuid-rm-web.ofc.kobe-u.ac.jp/profile/ja.f71dc41c0403f789520e17560c007669.html / / / / / / / / / / / / https://kuid-rm-web.ofc.kobe-u.ac.jp/profile/ja.435041c825fb64d4520e17560c007669.html
データ登録日 2020-03-09
