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https://hdl.handle.net/20.500.14094/90004467
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2024-05-03
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90004467 (fulltext)
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メタデータID
90004467
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open access
出版タイプ
Version of Record
タイトル
Broad-spectrum antiviral agents: secreted phospholipase A(2) targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane
著者
Chen, Ming ; Aoki-Utsubo, Chie ; Kameoka, Masanori ; Deng, Lin ; Terada, Yutaka ; Kamitani, Wataru ; Sato, Kei ; Koyanagi, Yoshio ; Hijikata, Makoto ; Shindo, Keiko ; Noda, Takeshi ; Kohara, Michinori ; Hotta, Hak
著者ID
A0153
研究者ID
1000090817074
著者名
Chen, Ming
陳, 明
チン, メイ
所属機関名
保健学研究科
著者名
Aoki-Utsubo, Chie
著者ID
A0045
研究者ID
1000060281838
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=01cae9e1f2290a00520e17560c007669
著者名
Kameoka, Masanori
亀岡, 正典
カメオカ, マサノリ
所属機関名
保健学研究科
著者ID
A0830
研究者ID
1000040437497
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=e256bdfcb1f5fc23520e17560c007669
著者名
Deng, Lin
鄧, 琳
トウ, リン
所属機関名
医学研究科
著者名
Terada, Yutaka
著者名
Kamitani, Wataru
著者名
Sato, Kei
著者名
Koyanagi, Yoshio
著者名
Hijikata, Makoto
著者名
Shindo, Keiko
著者名
Noda, Takeshi
著者名
Kohara, Michinori
著者ID
A0749
研究者ID
1000040116249
著者名
Hotta, Hak
堀田, 博
ホツタ, ハク
所属機関名
保健学研究科
収録物名
Scientific Reports
巻(号)
7
ページ
15931-15931
出版者
Nature Publishing Group
刊行日
2017-11-21
公開日
2018-01-10
抄録
Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae. Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The phospholipid content of the ER membrane differs from that of the plasma membrane (PM). The phospholipase A(2) (PLA(2)) superfamily consists of a large number of members that specifically catalyse the hydrolysis of phospholipids at a particular position. Here we show that the CM-II isoform of secreted PLA(2) obtained from Naja mossambica mossambica snake venom (CM-II-sPLA(2)) possesses potent virucidal (neutralising) activity against HCV, DENV and JEV, with 50% inhibitory concentrations (IC50) of 0.036, 0.31 and 1.34 ng/ ml, respectively. In contrast, the IC50 values of CM-II-sPLA(2) against viruses that bud through the PM (Sindbis virus, influenza virus and Sendai virus) or trans-Golgi network (TGN) (herpes simplex virus) were > 10,000 ng/ml. Moreover, the 50% cytotoxic (CC50) and haemolytic (HC50) concentrations of CMII- sPLA(2) were > 10,000 ng/ml, implying that CM-II-sPLA(2) did not significantly damage the PM. These results suggest that CM-II-sPLA(2) and its derivatives are good candidates for the development of broadspectrum antiviral drugs that target viral envelope lipid bilayers derived from the ER membrane.
カテゴリ
医学研究科
保健学研究科
学術雑誌論文
権利
© The Author(s) 2017
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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資源タイプ
journal article
言語
English (英語)
eISSN
2045-2322
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関連情報
DOI
https://doi.org/10.1038/s41598-017-16130-w
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