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https://hdl.handle.net/20.500.14094/90004544
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2024-05-03
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90004544 (fulltext)
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メタデータID
90004544
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.
著者
Miyaji, Naomi ; Shimizu, Motoki ; Miyazaki, Junji ; Osabe, Kenji ; Sato, Maho ; Ebe, Yusuke ; Takada, Satoko ; Kaji, Makoto ; Dennis, Elizabeth S. ; Fujimoto, Ryo ; Okazaki, Keiichi
著者名
Miyaji, Naomi
著者名
Shimizu, Motoki
著者名
Miyazaki, Junji
著者名
Osabe, Kenji
著者名
Sato, Maho
著者名
Ebe, Yusuke
著者名
Takada, Satoko
著者名
Kaji, Makoto
著者名
Dennis, Elizabeth S.
著者ID
A0337
研究者ID
1000060620375
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=2d794652078d165f520e17560c007669
著者名
Fujimoto, Ryo
藤本, 龍
フジモト, リョウ
所属機関名
農学研究科
著者名
Okazaki, Keiichi
収録物名
Plant Cell Reports
巻(号)
36(12)
ページ
1841-1854
出版者
Springer
刊行日
2017-12
公開日
2019-01-01
抄録
Resistant and susceptible lines in Brassica rapa have different immune responses against Fusarium oxysporum inoculation. Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to Foc is still unknown. By comparing with and without Foc inoculation in both resistant and susceptible lines of Chinese cabbage (Brassica rapa var. pekinensis), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by Foc inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that Foc inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between B. rapa and Arabidopsis thaliana after F. oxysporum inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and A. thaliana. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against F. oxysporum.
キーワード
Fusarium yellows
Chinese cabbage
Transcriptome
Systemic acquired resistance
Brassica rapa
Fusarium oxysporum f. sp conglutinans
カテゴリ
農学研究科
学術雑誌論文
権利
© Springer-Verlag GmbH Germany 2017 This is a post-peer-review, pre-copyedit version of an article published in Plant Cell Reports. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00299-017-2198-9
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資源タイプ
journal article
言語
English (英語)
ISSN
0721-7714
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eISSN
1432-203X
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関連情報
DOI
https://doi.org/10.1007/s00299-017-2198-9
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