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https://hdl.handle.net/20.500.14094/90004561
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2024-04-30
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90004561 (fulltext)
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メタデータID
90004561
アクセス権
open access
出版タイプ
Version of Record
タイトル
The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture
著者
Watanabe, Daisuke ; Koyanagi-Aoi, Michiyo ; Taniguchi-Ikeda, Mariko ; Yoshida, Yukiko ; Azuma, Takeshi ; Aoi, Takashi
著者名
Watanabe, Daisuke
著者ID
A0443
研究者ID
1000090432327
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=5963935ba79ebd9a520e17560c007669
著者名
Koyanagi-Aoi, Michiyo
青井, 三千代
アオイ, ミチヨ
所属機関名
医学部附属病院
著者ID
A0473
研究者ID
1000000410738
著者名
Taniguchi-Ikeda, Mariko
池田, 真理子
イケダ, マリコ
所属機関名
医学研究科
著者名
Yoshida, Yukiko
著者ID
A0058
研究者ID
1000060221040
著者名
Azuma, Takeshi
東, 健
アズマ, タケシ
所属機関名
医学研究科
著者ID
A0444
研究者ID
1000000546997
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=fc332006706c5b9e520e17560c007669
著者名
Aoi, Takashi
青井, 貴之
アオイ, タカシ
所属機関名
科学技術イノベーション研究科
収録物名
Stem Cells Translational Medicine
巻(号)
7(1)
ページ
34-44
出版者
Wiley
刊行日
2018-01
公開日
2018-01-30
抄録
γδT cells constitute a small proportion of lymphocytes in peripheral blood. Unlike αβT cells, the anti-tumor activities are exerted through several different pathways in a MHC-unrestricted manner. Thus, immunotherapy using γδT cells is considered to be effective for various types of cancer. Occasionally, however, ex vivo expanded cells are not as effective as expected due to cell exhaustion. To overcome the issue of T-cell exhaustion, researchers have generated induced pluripotent stem cells (iPSCs) that harbor the same T-cell receptor (TCR) genes as their original T-cells, which provide nearly limitless sources for antigen-specific cytotoxic T lymphocytes (CTLs). However, these technologies have focused on αβT cells and require a population of antigen-specific CTLs, which are purified by cell sorting with HLA-peptide multimer, as the origin of iPS cells. In the present study, we aimed to develop an efficient and convenient system for generating iPSCs that harbor rearrangements of the TCRG and TCRD gene regions (γδT-iPSCs) without cell-sorting. We stimulated human whole peripheral blood mononuclear cell (PBMC) culture using Interleukin-2 and Zoledronate to activate γδT cells. Gene transfer into those cells with the Sendai virus vector resulted in γδT cell-dominant expression of exogenous genes. The introduction of reprogramming factors into the stimulated PBMC culture allowed us to establish iPSC lines. Around 70% of the established lines carried rearrangements at the TCRG and TCRD gene locus. The γδT-iPSCs could differentiate into hematopoietic progenitors. Our technology will pave the way for new avenues toward novel immunotherapy that can be applied for various types of cancer.
キーワード
Induced pluripotent stem cells
T-lymphocytes
gamma-delta TCR
immunotherapy
カテゴリ
医学研究科
医学部附属病院
科学技術イノベーション研究科
学術雑誌論文
権利
© 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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資源タイプ
journal article
言語
English (英語)
ISSN
2157-6564
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eISSN
2157-6580
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関連情報
DOI
https://doi.org/10.1002/sctm.17-0021
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