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https://hdl.handle.net/20.500.14094/90005386
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2024-04-29
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90005386 (fulltext)
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メタデータID
90005386
アクセス権
open access
出版タイプ
Version of Record
タイトル
Usefulness of environmental DNA for detecting Schistosoma mansoni occurrence sites in Madagascar
著者
Sato, Marcello Otake ; Rafalimanantsoa, Armand ; Ramarokoto, Charles ; Rahetilahy, Alain Marcel ; Ravoniarimbinina, Pascaline ; Kawai, Satoru ; Minamoto, Toshifumi ; Sato, Megumi ; Kirinoki, Masashi ; Rasolofo, Voahangy ; De Calan, Mathilde ; Chigusa, Yuichi
著者名
Sato, Marcello Otake
著者名
Rafalimanantsoa, Armand
著者名
Ramarokoto, Charles
著者名
Rahetilahy, Alain Marcel
著者名
Ravoniarimbinina, Pascaline
著者名
Kawai, Satoru
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
著者名
Sato, Megumi
著者名
Kirinoki, Masashi
著者名
Rasolofo, Voahangy
著者名
De Calan, Mathilde
著者名
Chigusa, Yuichi
収録物名
International Journal of Infectious Diseases
巻(号)
76
ページ
130-136
出版者
Elsevier
刊行日
2018-11
公開日
2018-11-21
抄録
Objectives: Schistosomiasis is an important disease in Madagascar, and several studies on the disease have focused on the occurrence of the parasite in humans. However, the range of the pathogen in the environment and its impact on human infection is difficult to predict. An environmental DNA (eDNA) detection system for Schistosoma mansoni was developed to improve schistosomiasis eco-epidemiology studies. Methods: Primers and probes were designed and tested in experimental biotopes. The field study was conducted in Maevatanana District of Madagascar. Seven water sources with human use were sampled, with a total of 21 water samples collected. Snails were collected, and patients were examined by ultrasound to determine the occurrence of schistosomiasis in the study area. Results: One water source with active transmission was identified through the detection of S. mansoni eDNA in the water and the intermediate host Biomphalaria pfeifferi collected from the same water source. People with clinical schistosomiasis were found in the area, reinforcing the findings. Conclusions: The application of eDNA in eco-epidemiology enables the determination of hot spots and safe spots in endemic areas, constituting an alternative ecological tool for follow-up and monitoring of control programs for schistosomiasis, and contributing information on water safety for improving the standard of living of the people in endemic areas.
キーワード
Eco-epidemiology
EcoHealth
One Health
Environment
Schistosomiasis
Africa
Madagascar
eDNA
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2018 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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資源タイプ
journal article
言語
English (英語)
ISSN
1201-9712
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eISSN
1878-3511
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関連情報
DOI
https://doi.org/10.1016/j.ijid.2018.08.018
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