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https://hdl.handle.net/20.500.14094/90005786
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2024-04-29
06:29 集計
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90005786 (fulltext)
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メタデータID
90005786
アクセス権
open access
出版タイプ
Version of Record
タイトル
Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution
著者
Jo, Toshiaki ; Murakami, Hiroaki ; Yamamoto, Satoshi ; Masuda, Reiji ; Minamoto, Toshifumi
著者名
Jo, Toshiaki
著者名
Murakami, Hiroaki
著者ID
A0212
研究者ID
1000010643257
著者名
Yamamoto, Satoshi
山本, 哲史
ヤマモト, サトシ
所属機関名
人間発達環境学研究科
著者名
Masuda, Reiji
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
収録物名
Ecology and Evolution
巻(号)
9(3)
ページ
1135-1146
出版者
Wiley
刊行日
2019-02
公開日
2019-04-05
抄録
Environmental DNA (eDNA) analysis has successfully detected organisms in various aquatic environments. However, there is little basic information on eDNA, including the eDNA shedding and degradation processes. This study focused on water temperature and fish biomass and showed that eDNA shedding, degradation, and size distribution varied depending on water temperature and fish biomass. The tank experiments consisted of four temperature levels and three fish biomass levels. The total eDNA and size-fractioned eDNA from Japanese Jack Mackerels (Trachurus japonicus) were quantified before and after removing the fish. The results showed that the eDNA shedding rate increased at higher water temperature and larger fish biomass, and the eDNA decay rate also increased at higher temperature and fish biomass. In addition, the small-sized eDNA fractions were proportionally larger at higher temperatures, and these proportions varied among fish biomass. After removing the fish from the tanks, the percentage of eDNA temporally decreased when the eDNA size fraction was >10 mu m, while the smaller size fractions increased. These results have the potential to make the use of eDNA analysis more widespread in the future.
キーワード
decay rate
environmental DNA
fish biomass
Japanese Jack Mackerel (Trachurus japonicus)
quantitative real-time PCR
shedding rate
size distribution
water temperature
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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資源タイプ
journal article
言語
English (英語)
eISSN
2045-7758
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関連情報
DOI
https://doi.org/10.1002/ece3.4802
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