神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90006402
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2024-05-04
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90006402 (fulltext)
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7.85 MB
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メタデータID
90006402
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Roles of Cdc42 and Rac in Bergmann glia during cerebellar corticogenesis
著者
Sakamoto, Isao ; Ueyama, Takehiko ; Hayashibe, Masakazu ; Nakamura, Takashi ; Mohri, Hiroaki ; Kiyonari, Hiroshi ; Shigyo, Michiko ; Tohda, Chihiro ; Saito, Naoaki
著者名
Sakamoto, Isao
著者ID
A1239
研究者ID
1000080346254
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=443e05a0c3fc0358520e17560c007669
著者名
Ueyama, Takehiko
上山, 健彦
ウエヤマ, タケヒコ
所属機関名
バイオシグナル総合研究センター
著者名
Hayashibe, Masakazu
著者名
Nakamura, Takashi
著者名
Mohri, Hiroaki
著者名
Kiyonari, Hiroshi
著者名
Shigyo, Michiko
著者名
Tohda, Chihiro
著者ID
A0754
研究者ID
1000060178499
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=5821517134b6cd81520e17560c007669
著者名
Saito, Naoaki
齋藤, 尚亮
サイトウ, ナオアキ
所属機関名
バイオシグナル総合研究センター
収録物名
Experimental Neurology
巻(号)
302
ページ
57-67
出版者
Elsevier
刊行日
2018-04
公開日
2019-10-17
抄録
Bergmann glia (BG) are important in the inward type of radial migration of cerebellar granule neurons (CGNs). However, details regarding the functions of Cdc42 and Rac in BG for radial migration of CGN are unknown. To examine the roles of Cdc42 and Rac in BG during cerebellar corticogenesis, mice with a single deletion of Cdc42 or Rac1 and those with double deletions of Cdc42 and Rac1 under control of the glial fibrillary acidic protein (GFAP) promoter: GFAP-Cre;Cdc42flox/flox (Cdc42-KO), GFAP–Cre;Rac1flox/flox (Rac1-KO), and GFAP-Cre; Cdc42 flox/flox;Rac1flox/flox (Cdc42/Rac1-DKO) mice, were generated. Both Cdc42-KO and Rac1-KO mice, but more obviously Cdc42-KO mice, had disturbed alignment of BG in the Purkinje cell layer (PCL). We found that Cdc42-KO, but not Rac1-KO, induced impaired radial migration of CGNs in the late phase of radial migration, leading to retention of CGNs in the lower half of the molecular layer (ML). Cdc42-KO, but not Rac1-KO, mice also showed aberrantly aligned Purkinje cells (PCs). These phenotypes were exacerbated in Cdc42/Rac1-DKO mice. Alignment of BG radial fibers in the ML and BG endfeet at the pial surface of the cerebellum evaluated by GFAP staining was disturbed and weak in Cdc42/Rac1-DKO mice, respectively. Our data indicate that Cdc42 and Rac, but predominantly Cdc42, in BG play important roles during the late phase of radial migration of CGNs. We also report here that Cdc42 is involved in gliophilic migration of CGNs, in contrast to Rac, which is more closely connected to regulating neurophilic migration.
キーワード
Astrocytes
Cerebellar development
Cerebellar granule neurons
Gliophilic migration
Neuregulin 1
Notch3
Radial migration
Rho-family small GTPases
カテゴリ
バイオシグナル総合研究センター
学術雑誌論文
権利
© 2018 Elsevier B.V.
This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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資源タイプ
journal article
言語
English (英語)
ISSN
0014-4886
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NCID
AA00641190
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関連情報
DOI
https://doi.org/10.1016/j.expneurol.2017.12.003
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