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https://hdl.handle.net/20.500.14094/90007179
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2024-04-30
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90007179 (fulltext)
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90007179
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open access
出版タイプ
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タイトル
In vitro human colonic microbiota utilises D-β-hydroxybutyrate to increase butyrogenesis
著者
Sasaki, Kengo ; Sasaki, Daisuke ; Hannya, Asuka ; Tsubota, Jun ; Kondo, Akihiko
著者ID
A0278
研究者ID
1000050558301
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=543788ae48fc1b54520e17560c007669
著者名
Sasaki, Kengo
佐々木, 建吾
ササキ, ケンゴ
所属機関名
科学技術イノベーション研究科
著者ID
A0276
研究者ID
1000000650615
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=983adcb634cd583a520e17560c007669
著者名
Sasaki, Daisuke
佐々木, 大介
ササキ, ダイスケ
所属機関名
科学技術イノベーション研究科
著者名
Hannya, Asuka
著者名
Tsubota, Jun
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
収録物名
Scientific Reports
巻(号)
10(1)
ページ
8516-8516
出版者
Nature Research
刊行日
2020-05-22
公開日
2020-07-03
抄録
The ketone body D-β-hydroxybutyrate (DBHB) has gained attention owing to its cellular signalling function; however, its effect on the human colonic microbiota remains unclear. Here, DBHB dynamics in the human colon were investigated using an in vitro colonic microbiota model, which maintained most of the operational taxonomic units detected in the original faeces. Over 54% of 0.41% (w/v) DBHB was metabolised by microbiota models originating from seven faecal samples after 30h of fermentation (regarded as DBHB utilisers); however, <19% of DBHB was metabolised by microbiota models from five faecal samples (regarded as non-utilisers of DBHB). In utilisers, DBHB administration increased the relative abundance of the genus Coprococcus, correlated with increased butyrogenesis. Increased butyrogenesis was not observed in DBHB non-utilisers. Based on PICRUSt analysis, the relative abundance of β-hydroxybutyrate dehydrogenase was maintained in microbiota models from DBHB utilisers following DBHB administration; however, it decreased in microbiota models from non-utilisers. After 21h of fermentation, the intracellular glutamate concentration, which is indicative of growth, showed a positive correlation with DBHB utilisation (R2=0.70). Human colonic microbiotas with high growth activity demonstrate efficient utilisation of DBHB for increased butyrate production, which affords health benefits.
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科学技術イノベーション研究科
学術雑誌論文
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© The Author(s) 2020.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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資源タイプ
journal article
言語
English (英語)
eISSN
2045-2322
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関連情報
DOI
https://doi.org/10.1038/s41598-020-65561-5
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